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481 50:50 beamsplitter  (Thorlabs)
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Journal: Developmental cell

Article Title: Aging suppresses sphingosine-1-phosphate (S1P) chaperone ApoM in circulation resulting in maladaptive organ repair

doi: 10.1016/j.devcel.2020.05.024

Figure Lengend Snippet: Key Resources Table

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Organisms/Strains Mouse: Floxed S1pr1 ( Allende et al., 2003 ) RRID: MGI:2681963 Mouse: Apom transgenic ( Christoffersen et al., 2008 ) PMID: 18006500 Mouse: Apom knockout ( Christoffersen et al., 2008 ) PMID: 18006500 Mouse:Cdh5-Cre ERT2 ( Wang et al., 2010b ) RRID: MGI:3848984 Mouse: Albumin-Cre The Jackson Laboratory RRID: IMSR_JAX: 003574 Mouse: S1pr1-Gfp ( Kono et al., 2014 ) PMID: 24667638 Mouse: C57BL6/J The Jackson Laboratory N/A Bacterial and Viral Strains AAV9 ( Gao et al., 2004 ) PMID:15163731 Antibodies Rabbit polyclonal anti-Collagen I Abcam Cat#ab34710 Rabbit polyclonal anti-SMA Abcam Cat#ab5694 Rabbit polyclonal anti-desmin Abcam Cat#ab15200 Goat polyclonal anti-VE-Cadherin R&D Cat#AF1002 Rabbit polyclonal anti-SFTPC Abcam Cat#ab28744 Rabbit polyclonal anti-Aquaporin Abcam Cat#ab78486 Goat polyclonal anti-Podoplanin R&D Cat#AF3244 AF647 donkey anti-rabbit IgG Jackson ImmunoResearch Labs Cat#711-605-152 AF488 donkey anti-rabbit IgG Jackson ImmunoResearch Labs Cat#711-545-152 AF647 donkey anti-goat IgG Jackson ImmunoResearch Labs Cat#705-605-147 AF488 donkey anti-goat IgG Jackson ImmunoResearch Labs Cat#705-545-147 Deposited data RNA Seq raw data This paper GEO Software Image J 1.51 s NIH https://imagej.nih.gov/ij/ Graphpad Prism 8.0 Graphpad Software https://www.graphpad.com/scientificsoftware/prism/ Photoshop Adobe https://www.adobe.com/products/photoshop.html Recombinant DNA ApoM-Fc fusion construct ( Swendeman et al., 2017 ) PMID: 28811382 Chemicals, Peptides, and Recombinant Proteins Tamoxifen Sigma-Aldrich Cat#T5648 Corn Oil Sigma-Aldrich Cat#C8267 Collagenase I Roche Cat#11088793001 Dispase II Roche Cat#04942078001 RIPA Lysis Buffer Santa Cruz Cat#sc-364162 Phosphatase Inhibitor Cocktail Roche Cat#04906837001 Protease Inhibitor Cocktail Roche Cat#04693159001 Lipofectamine™ RNAiMAX Transfection Reagent Invitrogen Cat#13778150 EndoGRO-VEGF Complete Culture Media Kit Millipore Cat#SCME002 DMEM Gibco Cat#11965092 DAPI Invitrogen Cat#D1306 Ketamine Akorn NDC:59399-114-10 Xylazine Akorn NDC:59399-110-20 PFA Electron Microscopy Sciences Cat# 50-980-487 Hydrochloric acid Fisher Scientific Cat# A481-212 Tween80 Sigma-Aldrich Cat#P1754 ApoM-Fc ( Swendeman et al., 2017 ) PMID: 28811382 Critical Commercial Assays Mouse ApoM Elisa Kit Mlbio Biotechnology Cat# {"type":"entrez-nucleotide","attrs":{"text":"ML722506","term_id":"1767879886","term_text":"ML722506"}} ML722506 Hydroxyproline (Hyp) Kit Abcam Cat# ab222941 RNeasy Mini Kit QIAGEN Cat# 74104 EDU Proliferation Kit BD Pharmingen Cat# 565456 Oligonucleotides shRNA targeting sequence: Gankyrin 5’- CCGGG CAGCT TCGAA GAATA GGCAT CTCGA GATGC CTATT CTTCG AAGCT GCTTT TTG -3’ This paper N/A Open in a separate window Key Resources Table

Techniques: Transgenic Assay, Knock-Out, RNA Sequencing Assay, Software, Recombinant, Construct, Lysis, Protease Inhibitor, Transfection, Electron Microscopy, Enzyme-linked Immunosorbent Assay, shRNA, Sequencing